Article

Multiple sclerosis (MS) is considered a pathogenetic enigma.
Recently, efforts to implicate genetics in human susceptibility to MS have
identified an important role of mitochondrial DNA (mtDNA). G13708A is a common
mtDNA variation associated with MS in specific populations.
This study tested the
hypothesis that the mtDNA G13708A variation is associated with MS in an Iranian
population. METHODS:
Blood samples were collected from 100 MS
patients and 100 unrelated healthy controls. DNA was extracted using a
salting-out method, followed by polymerase chain reaction (PCR) amplification.
For assessment of restriction fragment length polymorphism (RFLP), PCR products
were restricted by restriction enzyme Mva I. Thereafter, the restriction products
were assessed by means of an ultraviolet (UV) transilluminator following
electrophoresis with 3% agarose gel. Accuracy of the genotyping procedure was
assessed by direct sequencing. RESULTS: The mtDNA G13708A variation was found in
17 cases (17%) and 19 controls (19%) (P=0.7, OR: 0.8, 95% CI: 0.3-1.9).
CONCLUSION: The findings of the present study fail to support the hypothesis that
the G13708A mtDNA variation is associated with MS in the selected Iranian
population.
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Langue : ANGLAIS